New approach for immobilization of 3-aminopropyltrimethoxysilane and TiO2 nanoparticles into cellulose for BJ1 skin cells proliferation

Naglaa Salem El-Sayed; Mohamed El-Sakhawy; Nicolas Brun; Peter Hesemann; Samir Kamel

doi:10.1016/j.carbpol.2018.07.004

New approach for immobilization of 3-aminopropyltrimethoxysilane and TiO₂ nanoparticles into cellulose for BJ1 skin cells proliferation

Carbohydr Polym. 2018 Nov 1:199:193-204. doi: 10.1016/j.carbpol.2018.07.004. Epub 2018 Jul 9.

Authors

Naglaa Salem El-Sayed¹, Mohamed El-Sakhawy², Nicolas Brun³, Peter Hesemann³, Samir Kamel²

Affiliations

¹ Cellulose & Paper Department, National Research Centre, 33 El-Bohouth St. Dokki, Giza, P.O. 12622, Egypt. Electronic address: [email protected].
² Cellulose & Paper Department, National Research Centre, 33 El-Bohouth St. Dokki, Giza, P.O. 12622, Egypt.
³ Institut Charles Gerhardt, UMR 5253 CNRS-UM-ENSCM, CMOS, Université de Montpellier, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France.

PMID: 30143120
DOI: 10.1016/j.carbpol.2018.07.004

Abstract

In the present study, tosylcellulose (TC) was used as a key intermediate for the selective coupling with 3-aminopropyltrimethoxysilane (APTMS) affording amino-propylsilane-grafted tosylcellulose (TC-Si). Solid state ¹³C NMR and FT-IR analyses confirmed the coupling and self-condensation of APTMS along TC. The changes in the surface morphology of the functionalized cellulose were identified by SEM imaging. The thermal stability of TC-Si was significantly improved as compared to MCC and TC. A new organic/inorganic hybrid cellulosic material was fabricated by embedding TiO₂ nanoparticles into TC-Si network. The new cellulose polymers were investigated for their ability to promote the proliferation of human skin fibroblast (BJ1). The cell cytotoxicity assay showed that both TC and TC-Si possessed moderate toxicity to BJ1 cells by 17% and 23.8%, respectively at 20 μM. Meanwhile, TC-Si/TiO₂ hybrid enhanced the proliferation of BJ1 by 42%. Additionally TC-Si/TiO₂ hybrid demonstrated promising antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans.

Keywords: 3-Aminopropyltrimethoxysilane; Antimicrobial activity; BJ1 fibroblast; Hybrid material; TiO(2) nanoparticles; Tosylcellulose.

MeSH terms

Anti-Bacterial Agents / chemical synthesis
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / toxicity
Anti-Infective Agents / chemical synthesis
Anti-Infective Agents / chemistry
Anti-Infective Agents / pharmacology*
Anti-Infective Agents / toxicity
Antifungal Agents / chemical synthesis
Antifungal Agents / chemistry
Antifungal Agents / pharmacology
Antifungal Agents / toxicity
Biocompatible Materials / chemical synthesis
Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology
Biocompatible Materials / toxicity
Candida albicans / drug effects
Cell Line
Cell Proliferation / drug effects*
Cellulose / analogs & derivatives*
Cellulose / chemical synthesis
Cellulose / pharmacology
Cellulose / toxicity
Fibroblasts / drug effects*
Humans
Nanoparticles / chemistry
Nanoparticles / toxicity
Porosity
Propylamines / chemistry
Propylamines / pharmacology*
Propylamines / toxicity
Pseudomonas aeruginosa / drug effects
Silanes / chemistry
Silanes / pharmacology*
Silanes / toxicity
Staphylococcus aureus / drug effects
Tissue Scaffolds / chemistry
Titanium / chemistry
Titanium / pharmacology*
Titanium / toxicity

Substances

Anti-Bacterial Agents
Anti-Infective Agents
Antifungal Agents
Biocompatible Materials
Propylamines
Silanes
3-aminopropyltrimethoxysilane
titanium dioxide
Cellulose
Titanium