The criteria for Diagnosis of Toxoplasmosis (WS/T 486-2015)（referred to as the Criteria） was compiled following the Management Measures for Health Criteria and GBT 1.1-2009 Standardization Working Guidelines. The Criteria is composed of six chapters, including the range of application, terms and definitions, diagnostic principle, diagnostic standard, and differential diagnosis. Four informative appendices (etiology, epidemiology, clinical manifestation, and differential diagnosis) and one normative appendix (laboratory examination) are appended. The Criteria was issued by the National Health and Family Planning Commission of the People’s Republic of China through No.21 of Chinese Health Announcement in 2015. The Criteria provides for the first time technical reference for diagnosis of toxoplasmosis in medical institutions and disease control institutions.