Selection of Antibody Fragments Against Structured DNA by Phage Display

Mahdi Zeraati; Marcel E Dinger; Daniel Christ

doi:10.1007/978-1-4939-8648-4_11

Selection of Antibody Fragments Against Structured DNA by Phage Display

Methods Mol Biol. 2018:1827:197-209. doi: 10.1007/978-1-4939-8648-4_11.

Authors

Mahdi Zeraati^{1

2}, Marcel E Dinger^{1

2}, Daniel Christ^{3

4}

Affiliations

¹ Garvan Institute of Medical Research, Sydney, NSW, Australia.
² Faculty of Medicine, St. Vincent's Clinical School, University of New South Wales, Sydney, NSW, Australia.
³ Garvan Institute of Medical Research, Sydney, NSW, Australia. [email protected].
⁴ Faculty of Medicine, St. Vincent's Clinical School, University of New South Wales, Sydney, NSW, Australia. [email protected].

PMID: 30196499
DOI: 10.1007/978-1-4939-8648-4_11

Abstract

In addition to the canonical B-form structure, DNA can adopt alternative conformations including Z DNA, triplex DNA, as well as G4 and i-Motif quadruplex structures. Such structures have been shown to form in cells in a dynamic manner. Monoclonal antibodies against such structures represent key tools to study the biological functions of these structures. Here we provide protocols for the generation of antibody fragments against structured DNA using phage display selections.

Keywords: Antibody fragment; G-quadruplex; Phage display; Secondary structure; Structured DNA; i-Motif.

MeSH terms

Antibodies / isolation & purification*
Bacteriophages / metabolism
Cell Surface Display Techniques / methods*
Clone Cells
DNA / metabolism*
Enzyme-Linked Immunosorbent Assay
Oligonucleotides / metabolism

Substances

Antibodies
Oligonucleotides
DNA