Two new C21 steroidal glycosides isolated from Cynanchum komarovii

Dan Zhao; Shan-Shan Su; Shao-Fei Chen; Xiao-Jie Lu; Gang Chen; Yu-Bo Wang; Guang-Yue Su; Yue-Hu Pei

doi:10.1016/S1875-5364(18)30098-0

Two new C₂₁ steroidal glycosides isolated from Cynanchum komarovii

Chin J Nat Med. 2018 Aug;16(8):610-614. doi: 10.1016/S1875-5364(18)30098-0.

Authors

Dan Zhao¹, Shan-Shan Su², Shao-Fei Chen¹, Xiao-Jie Lu¹, Gang Chen¹, Yu-Bo Wang³, Guang-Yue Su⁴, Yue-Hu Pei⁵

Affiliations

¹ Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
² Testing Laboratory, Qinghai Entry Exit Inspection and Quarantine Bureau, Xining 810000, China.
³ Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China.
⁴ Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: [email protected].
⁵ Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: [email protected].

PMID: 30197126
DOI: 10.1016/S1875-5364(18)30098-0

Abstract

The present study was designed to further investigate the C₂₁ steroidal glycosides in Cynanchum plants. Two new steroidal glycosides based on a 13, 14:14, 15-disecopregnane-type aglycone, komaroside P (1) and komaroside Q (2), together with three known compounds (3-5) were isolated from the whole herbs of Cynanchum komarovii. The aglycones of compounds 1 and 2 were two new disecopregnane. Their structures were elucidated on the basis of 1D, 2D NMR spectroscopic data and acid hydrolysis. All the compounds (1-5) showed potent inhibitory activities against human leukemia cell lines (HL-60) with IC₅₀ values ranging from 16.6 to 26.3 μmol·L^-1, compared to the positive control 5-fluorouracil (6.4 μmol·L^-1).

Keywords: C(21) steroidal glycosides; Cynanchum komarovii; Cytotoxic activity.

MeSH terms

Cell Survival / drug effects
Cynanchum / chemistry*
Drugs, Chinese Herbal / chemistry
Drugs, Chinese Herbal / isolation & purification
Drugs, Chinese Herbal / pharmacology
Glycosides / chemistry*
Glycosides / isolation & purification
Glycosides / pharmacology
HL-60 Cells
Humans
Magnetic Resonance Spectroscopy
Molecular Structure
Steroids / chemistry*
Steroids / isolation & purification
Steroids / pharmacology

Substances

Drugs, Chinese Herbal
Glycosides
Steroids