T cell receptor cross-reactivity expanded by dramatic peptide-MHC adaptability

Nat Chem Biol. 2018 Oct;14(10):934-942. doi: 10.1038/s41589-018-0130-4. Epub 2018 Sep 17.

Abstract

T cell receptor cross-reactivity allows a fixed T cell repertoire to respond to a much larger universe of potential antigens. Recent work has emphasized the importance of peptide structural and chemical homology, as opposed to sequence similarity, in T cell receptor cross-reactivity. Surprisingly, though, T cell receptors can also cross-react between ligands with little physiochemical commonalities. Studying the clinically relevant receptor DMF5, we demonstrate that cross-recognition of such divergent antigens can occur through mechanisms that involve heretofore unanticipated rearrangements in the peptide and presenting MHC protein, including binding-induced peptide register shifts and extensions from MHC peptide binding grooves. Moreover, cross-reactivity can proceed even when such dramatic rearrangements do not translate into structural or chemical molecular mimicry. Beyond demonstrating new principles of T cell receptor cross-reactivity, our results have implications for efforts to predict and control T cell specificity and cross-reactivity and highlight challenges associated with predicting T cell reactivities.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / chemistry
  • Autoimmunity
  • Cross Reactions
  • Crystallography, X-Ray
  • Epitopes / chemistry
  • Humans
  • Kinetics
  • Ligands
  • Molecular Mimicry
  • Oligopeptides / chemistry*
  • Protein Binding
  • Protein Domains
  • Receptors, Antigen, T-Cell / chemistry*
  • Retroviridae
  • Surface Plasmon Resonance
  • T-Lymphocytes / chemistry

Substances

  • Antigens
  • Epitopes
  • Ligands
  • MHC binding peptide
  • Oligopeptides
  • Receptors, Antigen, T-Cell