CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors

David L Hacker; Laurence Durrer; Soraya Quinche

doi:10.1007/978-1-4939-8730-6_9

CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors

Methods Mol Biol. 2018:1850:123-131. doi: 10.1007/978-1-4939-8730-6_9.

Authors

David L Hacker¹, Laurence Durrer², Soraya Quinche²

Affiliations

¹ Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. [email protected].
² Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

PMID: 30242684
DOI: 10.1007/978-1-4939-8730-6_9

Abstract

Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.

Keywords: CHO cells; Disposable bioreactors; HEK293 cells; Suspension cells; Transfection.

MeSH terms

Animals
Bioreactors*
CHO Cells
Cricetinae
Cricetulus
HEK293 Cells
Humans
Recombinant Proteins / genetics
Recombinant Proteins / metabolism*
Transfection

Substances

Recombinant Proteins