Objective: To establish an HPLC method for determining p-Anisidine in workplace air. Methods: An C(18) capillary column was used with the detection wavelength of 240 nm, the methanol solution (v/v=62:38) as mobile phase, the flow rate was 0.9 ml/min, and the injection volume was 20 μl. Results: The linear range (μg/ml) for p-Anisidine was 0~100; correlation coefficient was 0.999 7; the detection limit was 0.15 μg/ml; the average precision of RSD was 4.4%. The sampling efficiency was more than 95% using silicone tube as sampling filter. Sample can be stored at room temperature for more than 7 d. Conclusion: the method appered to be accurate, simple, sensitive and reliable, also have a high sampling efficiency, and can be usd to determine p-anisidine in workplace air simultaneously.
目的： 建立工作场所空气中对氨基苯甲醚的高效液相色谱检测法。 方法： 采用高效液相色谱法,色谱柱为C(18)柱，流动相为甲醇：纯水=62:38（v/v），流速0.9 ml/min，检测波长240 nm，进样体积20 μl。 结果： 该方法测定对氨基苯甲醚线性范围（μg/ml）为0~100；相关系数（r）0.999 7；最低检出限为0.15 μg/ml；平均精密度RSD为4.4 %。以硅胶管为采样滤料，采样效率>95 %。样品室温下可保存7 d以上。 结论： 该方法采样效率高，操作简便，精密度和灵敏度较高，可以满足工作场所空气中对氨基苯甲醚的测定。.
Keywords: High performance liquid; P-Anisidine; Workplace; chromatography.