High Rate of Ceftobiprole Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates from a Hospital in Central Italy

Antimicrob Agents Chemother. 2018 Nov 26;62(12):e01663-18. doi: 10.1128/AAC.01663-18. Print 2018 Dec.

Abstract

Ceftobiprole is a fifth-generation cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA). One-year surveillance at the Regional Hospital of Ancona (Italy) disclosed a 12% ceftobiprole resistance rate (12/102 isolates; MIC, ≥4 mg/liter). Epidemiological characterization demonstrated that the resistant isolates all belonged to different clones. Penicillin-binding protein (PBP) analysis showed substitutions in all PBPs and a novel insertion in PBP2a. The mecB and mecC genes were not detected. Ceftobiprole susceptibility screening is essential to avoid therapeutic failure and the spread of ceftobiprole-resistant strains.

Keywords: MRSA; ceftobiprole; cephalosporins; mecA gene; penicillin-binding protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Typing Techniques
  • Cephalosporins / pharmacology*
  • Drug Resistance, Multiple, Bacterial / drug effects*
  • Hospitals
  • Humans
  • Italy
  • Methicillin-Resistant Staphylococcus aureus / classification
  • Methicillin-Resistant Staphylococcus aureus / drug effects*
  • Methicillin-Resistant Staphylococcus aureus / genetics
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Mutation
  • Penicillin-Binding Proteins / genetics*
  • Staphylococcal Infections / microbiology

Substances

  • Anti-Bacterial Agents
  • Cephalosporins
  • Penicillin-Binding Proteins
  • ceftobiprole