Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level

Martin Černý; Miroslav Berka; Hana Habánová

doi:10.1007/978-1-4939-8814-3_6

Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level

Methods Mol Biol. 2019:1871:93-105. doi: 10.1007/978-1-4939-8814-3_6.

Authors

Martin Černý¹, Miroslav Berka², Hana Habánová²

Affiliations

¹ Faculty of AgriSciences, Department of Molecular Biology and Radiobiology, CEITEC - Central European Institute of Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, Czech Republic. [email protected].
² Faculty of AgriSciences, Department of Molecular Biology and Radiobiology, CEITEC - Central European Institute of Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, Czech Republic.

PMID: 30276734
DOI: 10.1007/978-1-4939-8814-3_6

Abstract

Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.

Keywords: C18; PEG; Peptide fractionation; Plant proteomics; Protein fractionation; SCX.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chemical Fractionation* / methods
Peptides / analysis
Peptides / isolation & purification
Plant Proteins / analysis
Plant Proteins / isolation & purification*
Proteome*
Proteomics* / methods
Solvents

Substances

Peptides
Plant Proteins
Proteome
Solvents