Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts

Stem Cell Res. 2018 Oct:32:135-138. doi: 10.1016/j.scr.2018.09.014. Epub 2018 Sep 19.

Abstract

Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage. Resource table.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cellular Reprogramming / genetics
  • Cellular Reprogramming / physiology*
  • Female
  • Fibroblasts / cytology*
  • Fibroblasts / metabolism*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Middle Aged
  • Skin / cytology*