Abstract
Altered expression of α-synuclein is linked to Parkinson's disease (PD). A major challenge to explore how the increased α-synuclein affect neurotoxicity is the lack of a suitable human neuronal cell model that mimics this scenario. Its expression in neural precursors affects their differentiation process, in addition to the neuronal adaptability and variability in maintaining a constant level of expression across passages. Here, we describe an SH-SY5Y line harboring Tet-ON SNCA cDNA cassette that allows for induction of controlled α-synuclein expression after neuronal differentiation, which can be an important tool for PD research.
Keywords:
Parkinson’s disease; dopaminergic neurons; inducible α-synuclein expressing neuronal model for PD; α-synuclein.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Cell Differentiation
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Cell Line, Tumor
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Choline O-Acetyltransferase / metabolism
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Doxycycline / pharmacology
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / genetics*
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Humans
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Neuroblastoma / pathology
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Neurons / drug effects
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Neurons / metabolism
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RNA, Messenger / metabolism
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Time Factors
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Transfection
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alpha-Synuclein / chemistry
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alpha-Synuclein / genetics
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alpha-Synuclein / metabolism*
Substances
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Anti-Bacterial Agents
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MAP2 protein, human
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Microtubule-Associated Proteins
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RNA, Messenger
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alpha-Synuclein
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Choline O-Acetyltransferase
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Doxycycline