Abstract
MicroRNAs (miRs) are small molecules important for regulation of transcription and translation. The objective was to identify hormonally regulated miRs in human endometrial stromal cells and to determine the impact of the endocrine disruptor, bisphenol A (BPA), on those miRs. miR microarray analysis and multiple confirmatory cell preparations treated with 17β-estradiol (E2) and BPA altered miR-27b, let-7c, let-7e and miR-181b. Further, decidualization downregulated miR-27b. VEGFB and VEGFC were validated as targets of miR-27b. Identification of miR-27b target genes suggests that BPA and E2 downregulate miR-27b thereby leading to upregulation of genes important for vascularization and angiogenesis of the endometrium during the menstrual cycle and decidualization.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Benzhydryl Compounds / pharmacology*
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Cells, Cultured
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Down-Regulation
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Endocrine Disruptors / pharmacology*
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Endometrium / blood supply*
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Endometrium / drug effects*
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Endometrium / metabolism
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Estradiol / pharmacology*
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Female
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Humans
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Menstrual Cycle / drug effects
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Menstrual Cycle / genetics
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Menstrual Cycle / metabolism
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Middle Aged
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Neovascularization, Physiologic / drug effects
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Phenols / pharmacology*
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Signal Transduction
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Stromal Cells / drug effects*
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Stromal Cells / metabolism
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Vascular Endothelial Growth Factor B / genetics
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Vascular Endothelial Growth Factor B / metabolism
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Vascular Endothelial Growth Factor C / genetics
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Vascular Endothelial Growth Factor C / metabolism
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Young Adult
Substances
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Benzhydryl Compounds
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Endocrine Disruptors
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MIRN27 microRNA, human
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MicroRNAs
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Phenols
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VEGFB protein, human
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VEGFC protein, human
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Vascular Endothelial Growth Factor B
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Vascular Endothelial Growth Factor C
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Estradiol
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bisphenol A