[IHC, FISH, CISH, NGS in non-small cell lung cancer: What changes in the biomarker era?]

C Hamard; X Mignard; N Pecuchet; N Mathiot; H Blons; P Laurent-Puig; K Leroy; A Lupo; J Chapron; F Giraud; J Arrondeau; F Goldwasser; M Alifano; D Damotte; M Wislez

doi:10.1016/j.pneumo.2018.09.013

[IHC, FISH, CISH, NGS in non-small cell lung cancer: What changes in the biomarker era?]

Rev Pneumol Clin. 2018 Oct;74(5):327-338. doi: 10.1016/j.pneumo.2018.09.013. Epub 2018 Oct 19.

[Article in French]

Authors

C Hamard¹, X Mignard², N Pecuchet³, N Mathiot⁴, H Blons³, P Laurent-Puig³, K Leroy⁵, A Lupo⁶, J Chapron⁴, F Giraud⁴, J Arrondeau⁷, F Goldwasser⁸, M Alifano⁹, D Damotte⁶, M Wislez¹⁰

Affiliations

¹ Service de pneumologie, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 27, rue du Faubourg-St-Jacques, 75014 Paris, France; Inserm UMRS1138, équipe « cancer, immune control and escape », centre de recherche des Cordeliers, Paris Descartes université, 75006 Paris, France.
² GRC n(o) 04, Theranoscan, Sorbonne université, hôpital Tenon, AP-HP, 75020 Paris, France.
³ Inserm UMRS 1147, université Paris Descartes-Sorbonne Paris Cité, hôpital européen Georges-Pompidou, Assistance publique-Hôpitaux de Paris, 75015 Paris, France.
⁴ Service de pneumologie, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 27, rue du Faubourg-St-Jacques, 75014 Paris, France.
⁵ Inserm UMRS1138, équipe « cancer, immune control and escape », centre de recherche des Cordeliers, Paris Descartes université, 75006 Paris, France.
⁶ Inserm UMRS1138, équipe « cancer, immune control and escape », centre de recherche des Cordeliers, Paris Descartes université, 75006 Paris, France; Service d'anatomie-pathologique, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 75014 Paris, France.
⁷ Service d'oncologie, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 75014 Paris, France.
⁸ Inserm UMRS1138, équipe « cancer, immune control and escape », centre de recherche des Cordeliers, Paris Descartes université, 75006 Paris, France; Service d'oncologie, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 75014 Paris, France.
⁹ Inserm UMRS1138, équipe « cancer, immune control and escape », centre de recherche des Cordeliers, Paris Descartes université, 75006 Paris, France; Service de chirurgie thoracique, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 75014 Paris, France.
¹⁰ Service de pneumologie, hôpital Cochin, Assistance publique-Hôpitaux de Paris, 27, rue du Faubourg-St-Jacques, 75014 Paris, France; Inserm UMRS1138, équipe « cancer, immune control and escape », centre de recherche des Cordeliers, Paris Descartes université, 75006 Paris, France. Electronic address: [email protected].

PMID: 30343945
DOI: 10.1016/j.pneumo.2018.09.013

Abstract

Lung cancer is the leading cause of cancer deaths in France, with about 30,000 deaths per year. The overwhelming majority (90 %) are tobacco-related. The prognosis is dark but great therapeutic advances have been made with the development of targeted therapies first and then immunotherapy afterwards. These medications are conditioned to the expression of biomarkers that require specific tools in routine to measure them. We will detail in this chapter several techniques of anatomopathology, cytogenetics and molecular biology necessary for the detection of biomarkers in lung cancers, and their applications in thoracic oncology in 2018.

Keywords: Anatomopathologie; Anatomopathology; Biologie moléculaire; Biomarkers; Biomarqueurs; Cancer du poumon; Cytogenetics; Cytogénétique; Lung cancer; Molecular biology.

Publication types

Review

MeSH terms

Biomarkers, Tumor / analysis*
Biomarkers, Tumor / metabolism
Carcinoma, Non-Small-Cell Lung / diagnosis*
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Non-Small-Cell Lung / metabolism
Carcinoma, Non-Small-Cell Lung / pathology
Chromatin Immunoprecipitation / methods
Cytogenetic Analysis / methods*
Cytogenetic Analysis / trends
High-Throughput Nucleotide Sequencing / methods*
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence / methods*
Lung Neoplasms / diagnosis*
Lung Neoplasms / genetics
Lung Neoplasms / metabolism
Lung Neoplasms / pathology
Sequence Analysis, DNA / methods
Translocation, Genetic

Substances

Biomarkers, Tumor