Low level of polymyxin resistance among nonclonal mcr-1-positive Escherichia coli from human sources in Brazil

Marcelo Pillonetto; Alana Mazzetti; Guilherme N Becker; Christian A Siebra; Lavinia N V S Arend; Afonso L Barth

doi:10.1016/j.diagmicrobio.2018.08.009

Low level of polymyxin resistance among nonclonal mcr-1-positive Escherichia coli from human sources in Brazil

Diagn Microbiol Infect Dis. 2019 Feb;93(2):140-142. doi: 10.1016/j.diagmicrobio.2018.08.009. Epub 2018 Aug 26.

Authors

Marcelo Pillonetto¹, Alana Mazzetti², Guilherme N Becker³, Christian A Siebra³, Lavinia N V S Arend³, Afonso L Barth⁴

Affiliations

¹ State Public Health Laboratory of Paraná, São José dos Pinhais, Brazil; Pontifical Catholic University of Paraná, Curitiba, Brazil; Instituto Nacional de Pesquisa em Resistência Antimicrobiana-INPRA, Brazil. Electronic address: [email protected].
² Pontifical Catholic University of Paraná, Curitiba, Brazil.
³ State Public Health Laboratory of Paraná, São José dos Pinhais, Brazil.
⁴ Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Instituto Nacional de Pesquisa em Resistência Antimicrobiana-INPRA, Brazil.

PMID: 30355469
DOI: 10.1016/j.diagmicrobio.2018.08.009

Abstract

We report 26 human isolates of mcr-1-positive Escherichia coli, most of them (65.4%) with a polymyxin B MIC of 2 mg/L. Seventeen out of the 24 mcr-1-positive E. coli proved to be nonclonal by rep-PCR which strengthens the hypothesis of environmental or animal origin of these strains and reinforces the one health context of antimicrobial resistance.

Keywords: Antimicrobial resistance; E. coli; One health; Polymyxin resistance; mcr-1.

MeSH terms

Anti-Bacterial Agents / pharmacology
Cohort Studies
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli Infections / microbiology*
Escherichia coli Proteins / genetics*
Humans
Microbial Sensitivity Tests
Polymyxins / pharmacology*

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
MCR-1 protein, E coli
Polymyxins