Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

Methods Mol Biol. 2019:1889:1-15. doi: 10.1007/978-1-4939-8897-6_1.

Abstract

Primary myoblasts derived from human tissue are a valuable tool in research of muscle disease and pathophysiology. However, skeletal muscle biopsies, especially from diseased muscle, contain a plethora of non-myogenic cells, necessitating purification of the myogenic cell population. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.

Keywords: CD56; CD82; Fluorescence-activated cell sorting (FACS); Immunostaining; Myoblast isolation; Pax7; Skeletal muscle; Tissue dissociation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers
  • Cell Differentiation
  • Cell Separation* / methods
  • Cells, Cultured
  • Flow Cytometry* / methods
  • Fluorescent Antibody Technique
  • Humans
  • Immunophenotyping
  • Muscle, Skeletal / cytology*
  • Myoblasts, Skeletal / cytology*
  • Myoblasts, Skeletal / metabolism*

Substances

  • Biomarkers