Discrimination of dried sea cucumber (Apostichopus japonicus) products from different geographical origins by sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS)-based proteomic analysis and chemometrics

Hongwei Zhang; Xiaomei Zhang; Xue Zhao; Jie Xu; Chao Lin; Ping Jing; Lingping Hu; Sa Zhao; Xuesong Wang; Bafang Li

doi:10.1016/j.foodchem.2018.08.082

Discrimination of dried sea cucumber (Apostichopus japonicus) products from different geographical origins by sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS)-based proteomic analysis and chemometrics

Food Chem. 2019 Feb 15:274:592-602. doi: 10.1016/j.foodchem.2018.08.082. Epub 2018 Aug 21.

Authors

Hongwei Zhang¹, Xiaomei Zhang², Xue Zhao³, Jie Xu⁴, Chao Lin⁵, Ping Jing⁵, Lingping Hu⁶, Sa Zhao⁵, Xuesong Wang⁶, Bafang Li⁷

Affiliations

¹ College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, PR China; Technical Center of Shandong Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70 Qutangxia Road, Qingdao, Shandong Province 266002, PR China.
² Technical Center of Shandong Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70 Qutangxia Road, Qingdao, Shandong Province 266002, PR China. Electronic address: [email protected].
³ College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, PR China. Electronic address: [email protected].
⁴ College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, PR China. Electronic address: [email protected].
⁵ Technical Center of Shandong Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70 Qutangxia Road, Qingdao, Shandong Province 266002, PR China.
⁶ College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
⁷ College of Food Science and Engineering, Ocean University of China, No. 5 Yu Shan Road, Qingdao, Shandong Province 266003, PR China. Electronic address: [email protected].

PMID: 30372983
DOI: 10.1016/j.foodchem.2018.08.082

Abstract

The article reports on discrimination of dried sea cucumber Apostichopus japonicus (A. japonicus) products from different geographical regions in China (Xiapu, Jiaonan, Weihai, Yantai, Dalian). For this purpose, sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS)-based proteomic analysis combined with chemometrics was evaluated. Proteome variations of A. japonicus samples determined by SWATH-MS using ultra-high performance liquid chromatography coupled to quadrupole time of flight mass spectrometer were mined by chemometric tools to clarify the correlation between protein quantitation data and geographical origin. A differentiating OPLS-DA model was constructed with 55 SWATH-MS quantified proteins. A. japonicus samples from five locations were clearly classified by the developed OPLS-DA model and 100% prediction accuracy was achieved in validation testing as well as real-life sample analysis. Seventeen proteins were identified as discriminatory biomarkers allowing geographical provenance. The proposed method might be applied in origin authentication of commercially dried A. japonicus products.

Keywords: A. japonicus; Chemometrics; Geographical origin; Proteomics; SWATH-MS.

MeSH terms

Animals
Biomarkers / chemistry
Chromatography, High Pressure Liquid
Desiccation*
Geography*
Informatics*
Mass Spectrometry*
Proteomics*
Stichopus / chemistry*

Substances

Biomarkers