[Effects of UV-C and of 8-MOP + UV-A on the T- and B-population of human lymphocytes in vitro (author's transl)]

Arch Dermatol Res (1975). 1977 Oct 27;260(1):63-70. doi: 10.1007/BF00558016.
[Article in German]

Abstract

The transformation of UV-C irradiated leucocytes and lymphocytes by the mitogens Con A, PHA and PWM is measured by the 3H-Tdr. incorporation after 72 h incubation. Furthermore T- and B-cells are determined by the method of rosette formation. A clear inhibition of the cell activity is seen after irradiation of leucocytes with 450 mJ/cm(2) and of a lymphocyte suspension with 5-10 mJ/cm(2). There are no significant differences between the effects of the various mitogens. The number of T-cells decreases proportionality to the various intensity, the number of B-cells remain constant. Irradiation with UV-A + 8-MOP cause, equally for all mitogens, a dosis dependent inhibition of thymidine incorporation.

Publication types

  • English Abstract

MeSH terms

  • B-Lymphocytes* / drug effects
  • B-Lymphocytes* / immunology
  • B-Lymphocytes* / radiation effects
  • Humans
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / radiation effects
  • Methoxsalen / pharmacology*
  • Rosette Formation
  • T-Lymphocytes* / drug effects
  • T-Lymphocytes* / immunology
  • T-Lymphocytes* / radiation effects
  • Ultraviolet Rays*

Substances

  • Methoxsalen