Anabaena sp. strain PCC 7120: Laboratory Maintenance, Cultivation, and Heterocyst Induction

Curr Protoc Microbiol. 2019 Feb;52(1):e71. doi: 10.1002/cpmc.71. Epub 2018 Nov 6.

Abstract

Anabaena sp. strain PCC 7120 is a multicellular, filamentous, freshwater cyanobacterium that is capable of differentiating specialized heterocyst cells for nitrogen fixation. This unit includes protocols for the growth and maintenance of Anabaena appropriate for a research or teaching laboratory. Controlled induction and assessment of heterocyst development is also covered. © 2018 by John Wiley & Sons, Inc.

Keywords: anabaena; cyanobacteria; heterocyst; photosynthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabaena / genetics
  • Anabaena / growth & development*
  • Anabaena / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Colony Count, Microbial / instrumentation
  • Colony Count, Microbial / methods*
  • Cryopreservation / methods*
  • Culture Media / metabolism
  • Gene Expression Regulation, Bacterial
  • Nitrogen Fixation
  • Staining and Labeling / methods*

Substances

  • Bacterial Proteins
  • Culture Media