Neuroprotective Efficacy of a Sigma 2 Receptor/TMEM97 Modulator (DKR-1677) after Traumatic Brain Injury

ACS Chem Neurosci. 2019 Mar 20;10(3):1595-1602. doi: 10.1021/acschemneuro.8b00543. Epub 2018 Dec 3.

Abstract

Compounds targeting the sigma 2 receptor, which we recently cloned and showed to be identical with transmembrane protein 97 (σ2R/TMEM97), are broadly applicable therapeutic agents currently in clinical trials for imaging in breast cancer and for treatment of Alzheimer's disease and schizophrenia. These promising applications coupled with our previous observation that the σ2R/TMEM97 modulator SAS-0132 has neuroprotective attributes and improves cognition in wild-type mice suggests that modulating σ2R/TMEM97 may also have therapeutic benefits in other neurodegenerative conditions such as traumatic brain injury (TBI). Herein, we report that DKR-1677, a novel derivative of SAS-0132 with increased affinity and selectivity for σ2R/Tmem97 ( Ki = 5.1 nM), is neuroprotective after blast-induced and controlled cortical impact (CCI) TBI in mice. Specifically, we discovered that treatment with DKR-1677 decreases axonal degeneration after blast-induced TBI and enhances survival of cortical neurons and oligodendrocytes after CCI injury. Furthermore, treatment with DKR-1677 preserves cognition in the Morris water maze after blast TBI. Our results support an increasingly broad role for σ2R/Tmem97 modulation in neuroprotection and suggest a new approach for treating patients suffering from TBI.

Keywords: Neuroprotection; blast injury; controlled cortical impact injury; traumatic brain injury; σ2R/TMEM97.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alzheimer Disease / drug therapy
  • Alzheimer Disease / metabolism
  • Animals
  • Brain Injuries, Traumatic / drug therapy*
  • Cognition / drug effects
  • Disease Models, Animal
  • Membrane Proteins / drug effects*
  • Neurons / drug effects
  • Neuroprotective Agents / pharmacology*
  • Receptors, sigma / drug effects*

Substances

  • Membrane Proteins
  • Neuroprotective Agents
  • Receptors, sigma
  • TMEM97 protein, human
  • sigma-2 receptor