rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent

P G Chistyakov; M N Abdukayumov; A G Veniaminova; S N Vladimirov; D M Graifer; S A Kazakov; G G Karpova

doi:10.1016/0014-5793(88)80324-7

rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent

FEBS Lett. 1988 Aug 15;236(1):246-50. doi: 10.1016/0014-5793(88)80324-7.

Authors

P G Chistyakov¹, M N Abdukayumov, A G Veniaminova, S N Vladimirov, D M Graifer, S A Kazakov, G G Karpova

Affiliation

¹ Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, USSR Academy of Sciences, Novosibirsk.

PMID: 3042466
DOI: 10.1016/0014-5793(88)80324-7

Abstract

The cleavable homobifunctional reagent dichloro[N,N,N',N'-tetrakis(2-aminoethyl)-1,6-hexamethylenediamminedi platinum (II)] dichloride was used for studying rRNA-protein cross-links in free 35S-labelled 70 S ribosomes and within initiation complex ribosome.AUGU6.fMet-tRNA(fMet). It was shown that the sets of proteins cross-linked to 16 S and 23 S rRNA in free 70 S ribosomes and in 70 S initiation complex do not differ significantly. The authors are the first to demonstrate most of the 23 S rRNA-protein cross-links and some 16 S rRNA-protein cross-links, in particular those with L7/L12 protein.

MeSH terms

Bacterial Proteins / metabolism*
Centrifugation, Density Gradient
Cross-Linking Reagents*
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics
Organoplatinum Compounds*
Peptide Chain Initiation, Translational
RNA, Ribosomal / metabolism*
RNA, Ribosomal, 16S / metabolism
RNA, Ribosomal, 23S / metabolism
Ribosomes / metabolism*

Substances

Bacterial Proteins
Cross-Linking Reagents
Organoplatinum Compounds
RNA, Ribosomal
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S
dichloro(N,N,N',N'-tetrakis(2-aminoethyl)-1,6-hexamethylenediamminediplatinum(II))