Inhibitory effect of Carnosol on UVB-induced inflammation via inhibition of STAT3

Arch Pharm Res. 2019 Mar;42(3):274-283. doi: 10.1007/s12272-018-1088-1. Epub 2018 Nov 15.

Abstract

Ultraviolet B (UVB) irradiation causes sunburn, inflammatory responses, dysregulation of immune function, oxidative stress, DNA damage and photocarcinogenesis on skin. Rosemary (Rosmarinus officinalis L.) has been reported to inhibit inflammation. Carnosol, a major component of Rosemary, has prominent anti-inflammatory effects. However, its protective effect on UVB-induced inflammatory skin responses has not yet been reported. Here, we investigated the effectiveness of carnosol on UVB-induced inflammation. We examined the anti-inflammation effect of topical application of carnosol (0.05 µg/cm2) on UVB (540 mJ/cm2, for 3 successive days)-induced skin inflammation in HR1 mice. Topical application of carnosol inhibited UVB-induced erythema, epidermal thickness, inflammatory responses in HR1 mice. Carnosol reduced the level of Immunoglobulin-E and IL-1β in blood serum of UVB-induced mice. Carnosol also significantly inhibited the UVB-induced expression of inflammatory marker protein (iNOS and COX-2) in back skin of mice. In addition, carnosol treated skin decreased activation of STAT3, a transcriptional factor regulating inflammatory genes. Our study suggested that carnosol has protective effects on skin inflammatory skin damages by UVB.

Keywords: Carnosol; Dermatitis; STAT3; UVB.

MeSH terms

  • Abietanes / pharmacology*
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin E / blood
  • Immunoglobulin E / metabolism
  • Inflammation / drug therapy*
  • Inflammation / metabolism
  • Mice
  • Mice, Transgenic
  • STAT3 Transcription Factor / antagonists & inhibitors*
  • STAT3 Transcription Factor / metabolism
  • Ultraviolet Rays*

Substances

  • Abietanes
  • Anti-Inflammatory Agents, Non-Steroidal
  • STAT3 Transcription Factor
  • Immunoglobulin E
  • carnosol