Abstract
Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
MeSH terms
-
Automation
-
Benzidines / metabolism
-
Diazonium Compounds / metabolism
-
Horseradish Peroxidase / metabolism*
-
Immunohistochemistry / methods*
-
Staining and Labeling / methods*
Substances
-
Benzidines
-
Diazonium Compounds
-
Fast Red B
-
benzidine
-
Horseradish Peroxidase
Grants and funding
The authors received no specific funding for this work. All authors are employed by Agilent Technologies. Agilent Technologies provided support in the form of salaries for all three authors, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.