Abstract
Immunotherapy is a developing but very promising arsenal to treat cancer. Acquiring a more potent and effective approach in cancer immunotherapy is always the ultimate pursuance. CTL-based therapies are highly acclaimed recently due to its direct killing property. However, difficulty in obtaining adequate number of CTLs is still a major obstacle. In previous studies, it is shown that pluripotent stem cell-derived cytotoxic T lymphocytes (CTL)-especially the genetically engineered tumor antigen-specific CTLs-may serve as a good candidate for this goal. Here we introduce a novel approach in generating tumor antigen-specific CTLs from induced pluripotent stem cells (iPSCs) by using both in vitro and in vivo priming mechanisms for the tumor management in a murine melanoma model.
Keywords:
Cytotoxic T lymphocytes; Immunotherapy; Melanoma; Notch signaling; Pluripotent stem cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Antigens, Neoplasm / immunology*
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Antigens, Neoplasm / metabolism
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Calcium-Binding Proteins
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods*
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Cell Differentiation / immunology
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Cell Line, Tumor
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Female
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Immunotherapy, Adoptive / methods*
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Induced Pluripotent Stem Cells / physiology
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / immunology
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Intercellular Signaling Peptides and Proteins / metabolism
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / immunology
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Intracellular Signaling Peptides and Proteins / metabolism
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Intramolecular Oxidoreductases / immunology
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Intramolecular Oxidoreductases / metabolism
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Melanoma, Experimental / immunology
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Melanoma, Experimental / pathology
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Melanoma, Experimental / therapy
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Receptors, Antigen, T-Cell / immunology
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Receptors, Antigen, T-Cell / metabolism
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Skin Neoplasms / immunology
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Skin Neoplasms / pathology
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Skin Neoplasms / therapy
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / metabolism
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T-Lymphocytes, Cytotoxic / transplantation
Substances
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Adaptor Proteins, Signal Transducing
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Antigens, Neoplasm
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Calcium-Binding Proteins
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DLL4 protein, mouse
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Dlk1 protein, mouse
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Intercellular Signaling Peptides and Proteins
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Receptors, Antigen, T-Cell
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Intramolecular Oxidoreductases
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dopachrome isomerase