Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure

J Clin Lab Anal. 2019 Mar;33(3):e22713. doi: 10.1002/jcla.22713. Epub 2018 Nov 29.

Abstract

Background: Chronic renal failure (CRF) has become a major public health concern, which increases the risk of stroke and systemic thromboembolism. Therefore, therapeutic strategies are in urgent requirement. This study was conducted for investigating efficacy of hemodialysis (HD), hemodiafiltration (HDF), and hemoperfusion (HP) in patients with CRF and the correlation with the presence of complications following HD therapy.

Methods: The therapeutic effect, living quality, biochemical indicators, and dry weight were detected before and after the treatment regimens. Flow cytometry was conducted to detect expressions of dendritic cell markers (CD40 and CD80) and platelet activation markers (CD62P and P10), and the relationship between their expression and therapeutic effect as well as the association of these expressions with complications was analyzed.

Results: After HD therapy, patients presented with decreased serum creatinine, serum phosphorus, triglyceride, parathyroid hormone, and β2 -MG expression; increased hemoglobin, plasma albumin expressions, and dry weight; and enhanced therapeutic effect and living quality. CD62P and P10 expressions decreased, while CD40 and CD80 expressions increased following HD therapy. The therapeutic effect improved in patients with low expressions of CD40 and CD80 and high expressions of CD62P and P10 following HP treatment and complications were lower after treatment of HDF and HP.

Conclusion: The aforementioned results indicated that CRF patients treated with HP exhibited higher expression of CD40 and CD80 and lower expression of CD62P and P10, suggesting that HP is conferred to have better efficacy than HDF and HD. Therefore, HP may be a promising clinical regimen for treatment of CRF patients.

Keywords: CD40; CD62P; CD80; chronic renal failure; hemodialysis.

MeSH terms

  • Adult
  • Aged
  • B7-1 Antigen / analysis
  • B7-1 Antigen / metabolism*
  • Biomarkers / analysis
  • Biomarkers / metabolism
  • CD40 Antigens / analysis
  • CD40 Antigens / metabolism*
  • Dendritic Cells / chemistry
  • Dendritic Cells / cytology
  • Female
  • Humans
  • Kidney Failure, Chronic* / blood
  • Kidney Failure, Chronic* / metabolism
  • Kidney Failure, Chronic* / therapy
  • Male
  • Middle Aged
  • P-Selectin / analysis
  • P-Selectin / metabolism*
  • Platelet Activation / physiology*
  • Renal Dialysis*

Substances

  • B7-1 Antigen
  • Biomarkers
  • CD40 Antigens
  • P-Selectin
  • SELP protein, human