Genome, Epigenome, and Transcriptome Editing via Chemical Modification of Nucleobases in Living Cells

Biochemistry. 2019 Feb 5;58(5):330-335. doi: 10.1021/acs.biochem.8b00958. Epub 2018 Dec 12.

Abstract

Base editors are tools that chemically modify the nucleobases of DNA and RNA in a programmable manner, allowing for genome, epigenome, and transcriptome editing in live cells. These tools can be used to introduce specific base transitions in DNA or RNA, manipulate methylation patterns in the epigenome, and create genetically encoded libraries in target genes. These various functions can be used to modulate every aspect of the central dogma. The efficiency and precision of base editors makes them useful in both basic research and the development of new therapies. Here we describe currently available base editors and the ways that they can be used to better understand and manipulate different aspects of the central dogma.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Epigenomics*
  • Gene Editing*
  • Genome*
  • Humans
  • Purines / chemistry*
  • Pyrimidines / chemistry*
  • Transcriptome*

Substances

  • Purines
  • Pyrimidines