Coregulatory long non-coding RNA and protein-coding genes in serum starved cells

Biochim Biophys Acta Gene Regul Mech. 2019 Jan;1862(1):84-95. doi: 10.1016/j.bbagrm.2018.11.004. Epub 2018 Nov 29.

Abstract

Serum starvation is widely used in cell biology to trigger cell cycle arrest, apoptosis, autophagy, and metabolic adaptations. Serum starvation-related molecular events have been well characterized at protein level but not at transcript level: how long non-coding RNAs contribute to the regulation of protein-coding genes is largely unknown. Here, we captured the lncRNA transcriptome in serum starved mouse embryonic fibroblasts and identified three main modes of action: cis-acting/coregulatory, trans-acting, and "miRNA-carrier". Whole-genome and individual gene level analyses support that our annotation provides an important platform for understanding lncRNA/protein-coding gene coregulatory mechanisms in serum starvation.

Keywords: Autophagy; H19X; Lockd; Miat; lncRNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Culture Techniques / methods
  • Culture Media, Serum-Free*
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Mice
  • MicroRNAs / biosynthesis
  • MicroRNAs / genetics
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • RNA, Long Noncoding / physiology
  • Transcriptional Activation
  • Transcriptome / genetics

Substances

  • Culture Media, Serum-Free
  • MicroRNAs
  • RNA, Long Noncoding