High-throughput single-cell transcriptomics on organoids

Agnieska Brazovskaja; Barbara Treutlein; J Gray Camp

doi:10.1016/j.copbio.2018.11.002

High-throughput single-cell transcriptomics on organoids

Curr Opin Biotechnol. 2019 Feb:55:167-171. doi: 10.1016/j.copbio.2018.11.002. Epub 2018 Nov 29.

Authors

Agnieska Brazovskaja¹, Barbara Treutlein², J Gray Camp³

Affiliations

¹ Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany.
² Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; Technical University Munich, 80333 Munich, Germany. Electronic address: [email protected].
³ Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig, Germany. Electronic address: [email protected].

PMID: 30504008
DOI: 10.1016/j.copbio.2018.11.002

Abstract

Three-dimensional (3D) tissues grown in culture from human stem cells offer the incredible opportunity to analyze and manipulate human development, and to generate patient-specific models of disease. Methods to sequence DNA and RNA in single cells are being used to analyze these so-called 'organoid' systems in high-resolution. Single-cell transcriptomics has been used to quantitate the similarity of organoid cells to primary tissue counterparts in the brain, intestine, liver, and kidney, as well as identify cell-specific responses to environmental variables and disease conditions. The merging of these two technologies, single-cell genomics and organoids, will have profound impact on personalized medicine in the near future.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

High-Throughput Screening Assays / methods*
Humans
Organ Specificity / genetics
Organoids / metabolism*
Precision Medicine
Single-Cell Analysis / methods*
Transcriptome / genetics*