Assessment of Maternal Cell Contamination in Prenatal Samples by Quantitative Fluorescent PCR (QF-PCR)

Christie M Buchovecky; Odelia Nahum; Brynn Levy

doi:10.1007/978-1-4939-8889-1_8

Assessment of Maternal Cell Contamination in Prenatal Samples by Quantitative Fluorescent PCR (QF-PCR)

Methods Mol Biol. 2019:1885:117-127. doi: 10.1007/978-1-4939-8889-1_8.

Authors

Christie M Buchovecky¹, Odelia Nahum¹, Brynn Levy²

Affiliations

¹ Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA.
² Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. [email protected].

PMID: 30506194
DOI: 10.1007/978-1-4939-8889-1_8

Abstract

When biopsying a fetal tissue like chorionic villi or amniotic fluid, there is a chance of getting some maternal material that could contaminate the fetal specimen and might lead to a misdiagnosis. Thus, all prenatal samples should be subjected to testing for maternal cell contamination. This is done using quantitative fluorescent PCR (QF-PCR) of short tandem repeat (STR) markers.

Keywords: Genotyping; Maternal cell contamination; Microsatellite markers; Prenatal diagnosis; Sample identity; Short tandem repeats.

MeSH terms

Amniotic Fluid
Chorionic Villi
DNA Contamination*
Data Analysis
Female
Humans
Microsatellite Repeats
Pregnancy
Prenatal Diagnosis / methods*
Prenatal Diagnosis / standards*
Real-Time Polymerase Chain Reaction / methods
Real-Time Polymerase Chain Reaction / standards*