SWI/SNF remains localized to chromatin in the presence of SCHLAP1

Jesse R Raab; Keriayn N Smith; Camarie C Spear; Carl J Manner; J Mauro Calabrese; Terry Magnuson

doi:10.1038/s41588-018-0272-z

SWI/SNF remains localized to chromatin in the presence of SCHLAP1

Nat Genet. 2019 Jan;51(1):26-29. doi: 10.1038/s41588-018-0272-z. Epub 2018 Dec 3.

Authors

Jesse R Raab¹, Keriayn N Smith¹, Camarie C Spear¹, Carl J Manner¹, J Mauro Calabrese², Terry Magnuson^{3

4}

Affiliations

¹ Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
² Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.
³ Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. [email protected].
⁴ Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. [email protected].

Abstract

SCHLAP1 is a long noncoding RNA that is reported to function by depleting the SWI/SNF complex from the genome. We investigated the hypothesis that SCHLAP1 affects only specific compositions of SWI/SNF. Using several assays, we found that SWI/SNF is not depleted from the genome by SCHLAP1 and that SWI/SNF is associated with many coding and noncoding RNAs, suggesting that SCHLAP1 may function in a SWI/SNF-independent manner.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Cell Line
Chromatin / genetics*
Chromosomal Proteins, Non-Histone / genetics*
Genome, Human / genetics
Humans
RNA, Long Noncoding / genetics*
Transcription Factors / genetics*

Substances

Chromatin
Chromosomal Proteins, Non-Histone
RNA, Long Noncoding
SChLAP1 long noncoding RNA
SWI-SNF-B chromatin-remodeling complex
Transcription Factors

Abstract

Publication types

MeSH terms

Substances

Grants and funding