Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer

Anal Chem. 2019 Jan 2;91(1):1157-1163. doi: 10.1021/acs.analchem.8b04935. Epub 2018 Dec 19.

Abstract

The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway of point-of-care analysis in clinical applications. In this work, a miniature mass spectrometry system was used for analysis of tissue samples. Direct tissue sampling coupled with extraction spray ionization was used with a home-built miniature mass spectrometer, Mini 12. Lipid species in tissue samples were well profiled in rat brain, kidney, and liver in a couple of minutes. By incorporating a photochemical (Paternò-Büchi) reaction, fast identification of lipid C═C location was realized. Relative quantitation of the lipid C═C isomer was performed by calculating the intensity ratio C═C diagnostic product ions, by which FA 18:1 (Δ9)/FA 18:1 (Δ11) was found to change significantly in mouse cancerous breast tissue samples. Accumulation of 2-hydroxylglutarate in human glioma samples, not in normal brains, can also be easily identified for rapid diagnosis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain
  • Breast
  • Fatty Acids / analysis*
  • Glioma / chemistry*
  • Glioma / diagnosis
  • Glutarates / analysis*
  • Humans
  • Kidney
  • Lipids / analysis*
  • Liver
  • Male
  • Mass Spectrometry
  • Point-of-Care Testing*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Fatty Acids
  • Glutarates
  • Lipids