Gene Expression Profiles of Tumor Necrosis Factor-α and Endothelin-1 in Obstructive Sleep Apnea

ORL J Otorhinolaryngol Relat Spec. 2019;81(1):16-23. doi: 10.1159/000490353. Epub 2018 Dec 11.

Abstract

Background/aims: The aim of this study was to observe the relationship between the gene expression profiles of tumor necrosis factor (TNF)-α and endothelin (EDN)-1 and obstructive sleep apnea (OSA).

Methods: A prospective, cross-sectional study performed at a tertiary-care academic center; 108 patients with snoring and day-time sleeplessness were included in this study carried out in the Otolaryngology Department. All patients were evaluated with 1-night polysomnography (PSG). There were 63 patients with OSA and 45 patients without OSA. In the OSA group, the median apnea hypopnea index (AHI) was 29.1; in the non-OSA group, the median AHI was 2.1. Blood samples were obtained from all 108 patients for the genetic analysis of the expression of TNF-α and EDN-1. PSG findings and gene expression levels were evaluated in both groups.

Results: The median (range) age was 46 (20-81) years, BMI 24.9 (15-49), EDN-1 gene expression 0.45 (0.02-67.88) pg/µL, and TNF-α gene expression 1.71 (0.08-59.52) pg/µL. We found that EDN-1 and TNF-α gene expression levels were significantly higher in the OSA group than in the control group (p = 0.009 vs. p < 0.001).

Conclusion: EDN-1 and TNF-α gene expression levels were associated with the occurrence of OSA.

Keywords: EDN-1; Gene expression; Obstructive sleep apnea; Polysomnography; TNF-α.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Body Mass Index
  • Case-Control Studies
  • Cross-Sectional Studies
  • Endothelin-1 / genetics*
  • Endothelin-1 / metabolism
  • Female
  • Humans
  • Male
  • Middle Aged
  • Polysomnography
  • Prospective Studies
  • RNA, Messenger / metabolism
  • Sleep Apnea, Obstructive / genetics*
  • Sleep Apnea, Obstructive / metabolism
  • Tumor Necrosis Factor-alpha / genetics*
  • Tumor Necrosis Factor-alpha / metabolism
  • Young Adult

Substances

  • Endothelin-1
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha