Engineered nanomaterials inhibit Podosphaera pannosa infection on rose leaves by regulating phytohormones

Yi Hao; Peihong Fang; Chuanxin Ma; Jason C White; Zhiqian Xiang; Haitao Wang; Zhao Zhang; Yukui Rui; Baoshan Xing

doi:10.1016/j.envres.2018.12.008

Engineered nanomaterials inhibit Podosphaera pannosa infection on rose leaves by regulating phytohormones

Environ Res. 2019 Mar:170:1-6. doi: 10.1016/j.envres.2018.12.008. Epub 2018 Dec 6.

Authors

Yi Hao¹, Peihong Fang², Chuanxin Ma³, Jason C White⁴, Zhiqian Xiang¹, Haitao Wang⁵, Zhao Zhang⁶, Yukui Rui⁷, Baoshan Xing⁸

Affiliations

¹ Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
² Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, China.
³ Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station, New Haven, CT 06504, United States; Stockbridge School of Agriculture, University of Massachusetts Amherst, Amherst, MA 01003, United States.
⁴ Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station, New Haven, CT 06504, United States.
⁵ Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, Heilongjiang 150070, China.
⁶ Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, China. Electronic address: [email protected].
⁷ Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address: [email protected].
⁸ Stockbridge School of Agriculture, University of Massachusetts Amherst, Amherst, MA 01003, United States.

PMID: 30554052
DOI: 10.1016/j.envres.2018.12.008

Abstract

In the present study, we investigated the antifungal effects of engineered nanomaterials (ENMs) against Podosphaera pannosa (P. pannosa), a fungal pathogen that causes powdery mildew on plants in the rose family. Four commercial ENMs, including multi-wall carbon nanotubes (MWCNTs), reduced graphene oxide (rGO), copper oxide (CuO) nanoparticles (NPs) and titanium dioxide (TiO₂) NPs, were used to prepare 50 or 200 mg/L NP suspensions in deionized water. Rose leaves in water-agar plates were sprayed by different ENM suspensions mixed with P. pannosa conidia. After a 19-day standard infection test, the growth of P. pannosa on rose leaves was evaluated. All four ENMs inhibited infection by P. pannosa at the concentration 200 mg/L, whereas only CuO NPs decreased fungal growth at 50 mg/L. The phytohormone content of the leaves was measured across all treatments to investigate potential ENMs antifungal mechanisms. The results suggest that ENMs increased plant resistance to fungal infection by altering the content of endogenous hormones, particularly zeatin riboside (ZR). Our study demonstrates that ENMs exhibited distinctly antifungal effects against P. pannosa on roses, and could be utilized as a novel plant protection strategy after a comprehensive assessment of potential environmental risk.

Keywords: Antifungal activity; Carbon-based nanomaterials; Metal-based nanoparticles; Powdery mildew; Roses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Infections
Nanostructures
Nanotubes, Carbon*
Plant Diseases / microbiology*
Plant Growth Regulators*
Plant Leaves / microbiology*
Rosa / microbiology*

Substances

Nanotubes, Carbon
Plant Growth Regulators