A Wars2 Mutant Mouse Model Displays OXPHOS Deficiencies and Activation of Tissue-Specific Stress Response Pathways

Cell Rep. 2018 Dec 18;25(12):3315-3328.e6. doi: 10.1016/j.celrep.2018.11.080.

Abstract

Mutations in genes essential for mitochondrial function have pleiotropic effects. The mechanisms underlying these traits yield insights into metabolic homeostasis and potential therapies. Here we report the characterization of a mouse model harboring a mutation in the tryptophanyl-tRNA synthetase 2 (Wars2) gene, encoding the mitochondrial-localized WARS2 protein. This hypomorphic allele causes progressive tissue-specific pathologies, including hearing loss, reduced adiposity, adipose tissue dysfunction, and hypertrophic cardiomyopathy. We demonstrate the tissue heterogeneity arises as a result of variable activation of the integrated stress response (ISR) pathway and the ability of certain tissues to respond to impaired mitochondrial translation. Many of the systemic metabolic effects are likely mediated through elevated fibroblast growth factor 21 (FGF21) following activation of the ISR in certain tissues. These findings demonstrate the potential pleiotropy associated with Wars2 mutations in patients.

Keywords: ISR; WARS2; adiposity; deafness; hypertrophic cardiomyopathy; mitochondrial dysfunction; pleiotropic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / pathology
  • Adipose Tissue, White / pathology
  • Adiposity
  • Alleles
  • Alternative Splicing / genetics
  • Animals
  • Base Sequence
  • Body Weight
  • Brain / pathology
  • Cardiomyopathy, Hypertrophic / blood
  • Cardiomyopathy, Hypertrophic / complications
  • Cardiomyopathy, Hypertrophic / physiopathology
  • Disease Models, Animal
  • Electron Transport
  • Evoked Potentials, Auditory, Brain Stem
  • Exons / genetics
  • Fibroblast Growth Factors / blood
  • Fibroblasts / metabolism
  • Hearing Loss / blood
  • Hearing Loss / complications
  • Hearing Loss / genetics
  • Hearing Loss / physiopathology
  • Mice
  • Mice, Mutant Strains
  • Muscle, Skeletal / metabolism
  • Mutation / genetics
  • Organ Specificity*
  • Organelle Biogenesis
  • Oxidative Phosphorylation*
  • Stress, Physiological*
  • Tryptophan-tRNA Ligase / genetics*
  • Tryptophan-tRNA Ligase / metabolism
  • Up-Regulation

Substances

  • fibroblast growth factor 21
  • Fibroblast Growth Factors
  • Tryptophan-tRNA Ligase
  • Wars2 protein, mouse