Dendrimer-Stabilized Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Multimode Imaging-Guided Combination Therapy of Tumors

Development of multifunctional theranostic nanoplatforms with improved diagnostic sensitivity and therapeutic efficiency of tumors still remains a great challenge. A unique multifunctional theranostic nanoplatform based on generation 5 (G5) poly(amidoamine) dendrimer-stabilized gold nanoflowers (NFs) embedded with ultrasmall iron oxide (USIO) nanoparticles (NPs) for multimode T ₁-weighted magnetic resonance (MR)/computed tomography (CT)/photoacoustic (PA) imaging-guided combination photothermal therapy (PTT) and radiotherapy (RT) of tumors is reported here. G5 dendrimer-stabilized Au NPs and citric acid-stabilized USIO NPs are separately prepared, the two particles under a certain Fe/Au molar ratio are mixed to form complexes, the complexes are exposed to Au growth solution to form NFs via a seed-mediated manner, and the remaining dendrimer terminal amines are acetylated. The formed dendrimer-stabilized Fe₃O₄/Au NFs (for short, Fe₃O₄/Au DSNFs) have a mean diameter of 99.8 nm, display good colloidal stability and cytocompatibility, and exhibit a near-infrared absorption feature. The unique structure and composition of the Fe₃O₄/Au DSNFs endows them with a high r ₁ relaxivity (3.22 mM^-1 s^-1) and photothermal conversion efficiency (82.7%), affording their uses as a theranostic nanoplatform for multimode MR/CT/PA imaging and combination PTT/RT of tumors with improved therapeutic efficacy, which is important for translational nanomedicine applications.