Abstract
Hepatitis C virus-mediated immune suppression is an underlying feature leading to the establishment of viral persistence and chronic infection. In particular, HCV core protein has been shown to exhibit significant immunosuppressive activity of T cells and antigen presenting cells. Using an HCV core transgenic mouse system, in which liver hepatocytes express core protein, it is possible to study the effects of core-mediated immune suppression in vivo during viral infection. In this protocol, we describe the procedures for evaluating antigen-specific CD8+ T cell responses in response to recombinant adenovirus infection in HCV core transgenic mice.
Keywords:
Antiviral T cells; CD8 T cells; Core protein; Flow cytometry; Hepatitis C virus; Liver; Liver mononuclear cell isolation; Tetramer.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenoviridae / genetics
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Animals
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CD8-Positive T-Lymphocytes / immunology*
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Cell Separation / instrumentation
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Cell Separation / methods
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Centrifugation, Density Gradient / instrumentation
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Centrifugation, Density Gradient / methods
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Flow Cytometry / instrumentation
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Flow Cytometry / methods
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Genetic Vectors / genetics
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Hepacivirus / immunology*
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Hepatitis C / immunology*
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Hepatitis C / virology
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Hepatocytes
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Leukocytes, Mononuclear
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Liver / cytology
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Liver / immunology
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Liver / virology
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Mice
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Mice, Transgenic
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Primary Cell Culture / instrumentation
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Primary Cell Culture / methods
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Transduction, Genetic / instrumentation
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Transduction, Genetic / methods
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Viral Core Proteins / immunology
Substances
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Viral Core Proteins
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nucleocapsid protein, Hepatitis C virus