Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis

Praseetha Prasannan; Navin Kumar Verma

doi:10.1007/978-1-4939-9036-8_7

Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis

Methods Mol Biol. 2019:1930:51-57. doi: 10.1007/978-1-4939-9036-8_7.

Authors

Praseetha Prasannan¹, Navin Kumar Verma²

Affiliations

¹ Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore. [email protected].
² Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.

PMID: 30610598
DOI: 10.1007/978-1-4939-9036-8_7

Abstract

The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.

Keywords: Chemoattractant; Chemotaxis; ICAM-1; LFA-1; SDF-1α; Transwell migration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Tracking / methods*
Cells, Cultured
Chemotaxis*
Cytokines / metabolism*
Electric Impedance
Humans
Intercellular Adhesion Molecule-1 / metabolism
Lymphocyte Activation*
Lymphocyte Function-Associated Antigen-1 / metabolism*
Signal Transduction
T-Lymphocytes / cytology
T-Lymphocytes / immunology
T-Lymphocytes / physiology*

Substances

Cytokines
ICAM1 protein, human
Lymphocyte Function-Associated Antigen-1
Intercellular Adhesion Molecule-1