Constitutive and inducible expression of cloned human interferon-b1 gene in HeLa cells through an episomal eukaryotic vector

M Giannì; A Gallina; G Barbanti-Brodano; G Milanesi

doi:10.1016/s0006-291x(88)80998-7

Constitutive and inducible expression of cloned human interferon-b1 gene in HeLa cells through an episomal eukaryotic vector

Biochem Biophys Res Commun. 1988 Dec 30;157(3):1175-81. doi: 10.1016/s0006-291x(88)80998-7.

Authors

M Giannì¹, A Gallina, G Barbanti-Brodano, G Milanesi

Affiliation

¹ Istituto di Genetica Biochimica ed Evoluzionistica CNR, Pavia, Italy.

PMID: 3061379
DOI: 10.1016/s0006-291x(88)80998-7

Abstract

An EcoRI fragment (1.83 Kb) of human DNA containing B1-interferon coding sequences was cloned in the episomal eukaryotic vector pBK-TK. The recombinant plasmid was transfected into thymidine kinase-deficient HeLa cells. Thymidine kinase-positive transformants were obtained and the cells were found to express B-interferon both constitutively and after induction with Newcastle Disease Virus or poly(rI)-poly(rC). The recombinant plasmid was present in stably transformed HeLa cells in an episomal state in hundreds of copies per cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Southern
Cloning, Molecular*
DNA, Recombinant
Gene Expression Regulation*
HeLa Cells / metabolism
Humans
Immunosorbent Techniques
Interferon Type I / analysis
Interferon Type I / biosynthesis
Interferon Type I / genetics*
Nucleic Acid Hybridization
Plasmids
RNA, Messenger / biosynthesis
Recombinant Proteins
Thymidine Kinase / genetics
Transcription, Genetic
Transfection
Transformation, Genetic

Substances

DNA, Recombinant
Interferon Type I
RNA, Messenger
Recombinant Proteins
Thymidine Kinase