Electrospun Nanofibers with Core-Shell Structure for Treatment of Bladder Regeneration

Tissue Eng Part A. 2019 Sep;25(17-18):1289-1299. doi: 10.1089/ten.TEA.2018.0255. Epub 2019 Mar 18.

Abstract

The extracellular matrix (ECM)-inspired electrospinning scaffolds have a good cytocompatibility. Hyaluronic acid (HA) is one of the main components in the bladder ECM, but it is hard to be made by electrospinning alone. In this study, it is shown that coaxial electrospinning can fabricate poly(l-lactide)/poly(e-caprolactone) (PLCL)/HA nanofibers with the core-shell structure. The HA coating nanofibers have more hydrophilicity, which can promote cell proliferation and migration by filopodial outgrowth. We also fabricated domed PLCL/HA nanofiber scaffolds used in bladder regeneration by improving bladder capacity and facilitating smooth muscle proliferation.

Keywords: bladder regeneration; cellular migration; cellular proliferation; coaxial electrospinning; hyaluronic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / physiology
  • Immunohistochemistry
  • Microscopy, Confocal
  • Microscopy, Electron, Transmission
  • Nanofibers / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Engineering / methods
  • Tissue Scaffolds / chemistry*
  • Urinary Bladder / cytology
  • Urinary Bladder / pathology*
  • Wettability