Dietary Extra-Virgin Olive Oil Polyphenols Do Not Attenuate Colon Inflammation in Transgenic HLAB-27 Rats but Exert Hypocholesterolemic Effects through the Modulation of HMGCR and PPAR-α Gene Expression in the Liver

Lifestyle Genom. 2018;11(2):99-108. doi: 10.1159/000495516. Epub 2019 Jan 10.

Abstract

Background: Human studies have demonstrated that olive oil phenolic compounds reduce inflammatory markers associated with chronic diseases.

Objectives: To explore the anti-inflammatory effects of extra-virgin olive oil polyphenols in an experimental model of inflammatory bowel disease (IBD).

Methods: HLA-B27 transgenic rats were fed an AIN-76 diet containing 10% corn oil (CO) or extra-virgin olive oil with high (EVOO) or low phenolic content (ROO) for 3 months. Wild-type rats (WT) were fed the CO diet.

Results: CO-fed HLA-B27 animals developed intestinal inflammation characterized by diarrhea, increased myeloperoxidase activity, and mucosal injury. None of these parameters were influenced by EVOO. Gene expression profiling indicated that proinflammatory pathways were upregulated in the colon mucosa of CO-fed HLA-B27 rats compared to WT, and this was further confirmed by RT-PCR for the iNOS, TNFα, and IL1β genes. EVOO significantly reduced TNFα gene expression in the colon mucosa and decreased total cholesterol blood levels compared to CO HLA-B27 rats (89.43 ± 3.66 vs. 111.5 ± 8.10 mg/dL, p < 0.05). This latter effect with EVOO was associated with reduced HMGCR and increased PPAR-α hepatic gene expression, compared to ROO.

Conclusion: These data indicate that olive oil polyphenols do not control colon inflammation in HLA-B27 transgenic rats but exert a positive effect on blood lipids by reducing total cholesterol levels. This preliminary result suggests the need to explore the efficacy of EVOO rich in polyphenols as a complementary strategy for managing hypercholesterolemia and to potentially limit statin-associated myotoxicity.

Keywords: Blood lipids; Colon inflammation; Inflammatory bowel disease; Olive oil; Polyphenols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticholesteremic Agents / pharmacology*
  • Colitis / complications
  • Colitis / genetics
  • Colitis / pathology*
  • Colon / drug effects
  • Colon / pathology
  • Dietary Fats / pharmacology
  • Gene Expression Regulation / drug effects
  • HLA-B27 Antigen / genetics
  • Hydroxymethylglutaryl CoA Reductases / genetics
  • Hydroxymethylglutaryl CoA Reductases / metabolism
  • Hypercholesterolemia / complications
  • Hypercholesterolemia / genetics
  • Hypercholesterolemia / prevention & control*
  • Inflammation / genetics
  • Inflammation / pathology
  • Inflammatory Bowel Diseases / complications
  • Inflammatory Bowel Diseases / genetics
  • Inflammatory Bowel Diseases / pathology
  • Liver / drug effects*
  • Liver / metabolism
  • Male
  • Olive Oil / pharmacology*
  • PPAR alpha / genetics
  • PPAR alpha / metabolism
  • Polyphenols / pharmacology*
  • Rats
  • Rats, Transgenic
  • Signal Transduction / drug effects
  • Signal Transduction / genetics

Substances

  • Anticholesteremic Agents
  • Dietary Fats
  • HLA-B27 Antigen
  • Olive Oil
  • PPAR alpha
  • Polyphenols
  • Hydroxymethylglutaryl CoA Reductases