Emission at 4.6 μm was observed from an N2O filled hollow core fiber laser. 8-ns pump pulses at 1.517 μm excited a vibrational overtone resulting in lasing on an R and P branch fundamental transition from the upper pump state. At optimum gas pressure of 80 Torr, photon conversion efficiency of 9% and slope efficiency of 3% were observed from a mirrorless laser. The laser threshold occurred at absorbed pump energy of 150 nJ in a 45-cm long fiber with 85 μm core diameter. The observed dependence of the laser output on gas pressure was shown to be a result of line broadening and relaxation rates.