Identification and Isolation of Regulatory B Cells in Mouse and Human

Madhvi Menon; Elizabeth C Rosser; Claudia Mauri

doi:10.1007/978-1-4939-8938-6_5

Identification and Isolation of Regulatory B Cells in Mouse and Human

Methods Mol Biol. 2019:1899:55-66. doi: 10.1007/978-1-4939-8938-6_5.

Authors

Madhvi Menon¹, Elizabeth C Rosser¹, Claudia Mauri²

Affiliations

¹ Centre for Rheumatology, Division of Medicine, University College London, 5 University Street, London, WC1E 6JF, UK.
² Centre for Rheumatology, Division of Medicine, University College London, 5 University Street, London, WC1E 6JF, UK. [email protected].

PMID: 30649765
DOI: 10.1007/978-1-4939-8938-6_5

Abstract

Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

Keywords: CD40; IL-10; Immature B cells; Inflammation; Regulatory B cells; T2-MZP B cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
B-Lymphocytes, Regulatory / cytology*
B-Lymphocytes, Regulatory / immunology
CD40 Antigens / immunology
Cell Separation / methods*
Humans
Interleukin-10 / immunology
Mice
T-Lymphocytes / cytology*
T-Lymphocytes / immunology

Substances

CD40 Antigens
Interleukin-10

Abstract

Publication types

MeSH terms

Substances

Grants and funding