The association of adelmidrol with sodium hyaluronate displays beneficial properties against bladder changes following spinal cord injury in mice

PLoS One. 2019 Jan 17;14(1):e0208730. doi: 10.1371/journal.pone.0208730. eCollection 2019.

Abstract

The disruption of coordinated control between the brain, spinal cord and peripheral nervous system caused by spinal cord injury (SCI) leads to several secondary pathological conditions, including lower urinary tract dysfunction. In fact, urinary tract dysfunction associated with SCI is urinary dysfunction could be a consequence of a lack of neuroregeneration of supraspinal pathways that control bladder function. The object of the current research was to explore the effects of adelmidrol + sodium hyaluronate, on bladder damage generated after SCI in mice. Spinal cord was exposed via laminectomy, and SCI was induced by extradural compression at T6 to T7 level, by an aneurysm clip with a closing force of 24 g. Mice were treated intravesically with adelmidrol + sodium hyaluronate daily for 48 h and 7 days after SCI. Adelmidrol + sodium hyaluronate reduced significantly mast cell degranulation and down-regulated the nuclear factor-κB pathway in the bladder after SCI both at 48 h and 7days. Moreover, adelmidrol + sodium hyaluronate reduced nerve growth factor expression, suggesting an association between neurotrophins and bladder pressure. At 7 days after SCI, the bladder was characterized by a marked bacterial infection and proteinuria; surprisingly, adelmidrol + sodium hyaluronate reduced significantly both parameters. These data show the protective roles of adelmidrol + sodium hyaluronate on bladder following SCI, highlighting a potential therapeutic target for the reduction of bladder changes.

MeSH terms

  • Animals
  • Blotting, Western
  • Dicarboxylic Acids / therapeutic use*
  • Fluorescent Antibody Technique
  • Hyaluronic Acid / therapeutic use*
  • Male
  • Mice
  • Nerve Growth Factor / metabolism
  • Palmitic Acids / therapeutic use*
  • Spinal Cord Injuries / drug therapy*
  • Spinal Cord Injuries / metabolism
  • Spinal Cord Injuries / physiopathology*
  • Urinary Bladder / drug effects*
  • Urinary Bladder / metabolism
  • Zonula Occludens-1 Protein / metabolism

Substances

  • Dicarboxylic Acids
  • Palmitic Acids
  • Zonula Occludens-1 Protein
  • adelmidrol
  • Hyaluronic Acid
  • Nerve Growth Factor

Grants and funding

The authors received no specific funding for this work.