Abstract
Roseoflavin (RoF), and its metabolic precursor 8-dimethylaminoriboflavin (AF), produced by Gram-positive bacteria Streptomyces davawensis and Streptomyces cinnabarinus, reveal a strong antibiotic effect against Staphyloccus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and other Gram-positive bacteria and could be promising for developing the new effective antibacterial drugs. In this work, we present the optimization of basic molecular tools for S. davawensis including total DNA preparation, PCR amplification of target gene, cell transformation. In addition, the compositions of cultivation media were studied and the optimal cultivation conditions for increased of RoF production have been developed.
MeSH terms
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / metabolism*
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Anti-Bacterial Agents / pharmacology
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Bacillus cereus / drug effects
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Bacillus cereus / growth & development
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Bacillus subtilis / drug effects
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Bacillus subtilis / growth & development
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Culture Media / chemistry
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Culture Media / pharmacology
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DNA, Bacterial / genetics*
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DNA, Bacterial / metabolism
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Genes, Bacterial*
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Microbial Sensitivity Tests
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Micrococcus luteus
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Plasmids / chemistry
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Plasmids / metabolism
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Polymerase Chain Reaction / methods
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Riboflavin / analogs & derivatives*
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Riboflavin / biosynthesis
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Riboflavin / isolation & purification
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Riboflavin / pharmacology
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / growth & development
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Streptomyces / drug effects
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Streptomyces / genetics*
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Streptomyces / metabolism
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Transformation, Genetic
Substances
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Anti-Bacterial Agents
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Culture Media
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DNA, Bacterial
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roseoflavin
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8-methylamino-riboflavin
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Riboflavin