[Improved and optimized preparation technology of agarose cell block]

M Q Gao; J J Shi; W W Liu; R Wan; X F Huang; A M Huang; W M Zhang

doi:10.3760/cma.j.issn.0529-5807.2019.02.017

[Improved and optimized preparation technology of agarose cell block]

Zhonghua Bing Li Xue Za Zhi. 2019 Feb 8;48(2):151-153. doi: 10.3760/cma.j.issn.0529-5807.2019.02.017.

[Article in Chinese]

Authors

M Q Gao¹, J J Shi², W W Liu³, R Wan³, X F Huang³, A M Huang³, W M Zhang³

Affiliations

¹ Research Centre of School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China; Department of Pathology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China.
² Research Centre of School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China; Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou 350122, China.
³ Department of Pathology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China.

PMID: 30695872
DOI: 10.3760/cma.j.issn.0529-5807.2019.02.017

Abstract

改良一种琼脂糖细胞块的制作方法，以获得细胞数量分布适中的细胞块，应用于培养肿瘤细胞的鉴定。选用平底离心管，以琼脂糖为介质制备细胞石蜡包埋块，并进行切片、HE染色、免疫组织化学染色。利用本方法制备的细胞块完整有形，HE染色效果好，免疫标记定位准确，背景清晰，结果稳定，可作为实验室常规的细胞块制备方法，并推广应用于病理的细胞学诊断。.