Stage-dependent differential effects of interleukin-1 isoforms on experimental atherosclerosis

Eur Heart J. 2019 Aug 7;40(30):2482-2491. doi: 10.1093/eurheartj/ehz008.

Abstract

Aims: Targeting interleukin-1 (IL-1) represents a novel therapeutic approach to atherosclerosis. CANTOS demonstrated the benefits of IL-1β neutralization in patients post-myocardial infarction with residual inflammatory risk. Yet, some mouse data have shown a prominent role of IL-1α rather than IL-1β in atherosclerosis, or even a deleterious effect of IL-1 on outward arterial remodelling in atherosclerosis-susceptible mice. To shed light on these disparate results, this study investigated the effect of neutralizing IL-1α or/and IL-1β isoforms starting either early in atherogenesis or later in ApoE-/- mice with established atheroma.

Methods and results: The neutralization of IL-1α or of both IL-1 isoforms impaired outward remodelling during early atherogenesis as assessed by micro-computed tomographic and histologic assessment. In contrast, the neutralization of IL-1β did not impair outward remodelling either during early atherogenesis or in mice with established lesions. Interleukin-1β inhibition promoted a slant of blood monocytes towards a less inflammatory state during atherogenesis, reduced the size of established atheromata, and increased plasma levels of IL-10 without limiting outward remodelling of brachiocephalic arteries.

Conclusion: This study established a pivotal role for IL-1α in the remodelling of arteries during early experimental atherogenesis, whereas IL-1β drives inflammation during atherogenesis and the evolution of advanced atheroma in mice.

Keywords: Arterial remodelling; Atherosclerosis; Inflammation; Interleukin-1.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arteritis / metabolism
  • Atherosclerosis / metabolism*
  • Disease Models, Animal
  • Interleukin-1alpha / antagonists & inhibitors
  • Interleukin-1alpha / blood
  • Interleukin-1alpha / metabolism*
  • Interleukin-1beta / antagonists & inhibitors
  • Interleukin-1beta / blood
  • Interleukin-1beta / metabolism*
  • Male
  • Mice
  • Mice, Knockout
  • Monocytes / metabolism
  • Plaque, Atherosclerotic / metabolism

Substances

  • IL1B protein, mouse
  • Il1a protein, mouse
  • Interleukin-1alpha
  • Interleukin-1beta