Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy

Histochem J. 1988 Sep;20(9):526-30. doi: 10.1007/BF01002651.

Abstract

We describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two Gram-positive bacteria, Streptococcus agalactiae and Streptococcus bovis. Treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin A allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein A-gold technique. Data obtained indicate that lectins are useful agents in preserving highly water-soluble capsular components during the electron microscopy procedures for both unembedded and embedded samples.

MeSH terms

  • Animals
  • Concanavalin A*
  • Immunohistochemistry
  • Lipopolysaccharides / analysis
  • Lipopolysaccharides / immunology
  • Microscopy, Electron / methods*
  • Preservation, Biological
  • Streptococcus agalactiae / analysis
  • Streptococcus agalactiae / ultrastructure*
  • Teichoic Acids / analysis
  • Teichoic Acids / immunology
  • Wheat Germ Agglutinins*

Substances

  • Lipopolysaccharides
  • Teichoic Acids
  • Wheat Germ Agglutinins
  • Concanavalin A
  • lipoteichoic acid