CD4CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques

J Med Primatol. 2019 Apr;48(2):82-89. doi: 10.1111/jmp.12399. Epub 2019 Feb 5.

Abstract

Background: Tuberculosis (TB) kills millions of people every year. CD4 and CD8 T cells are critical in the immune response against TB. T cells expressing both CD4 and CD8 (CD4CD8 T cells) are functionally active and have not been examined in the context of TB.

Methods: We examine peripheral blood mononuclear cells (PBMC) and bronchoalveolar lavage cells (BAL) and lung granulomas from 28 cynomolgus macaques during Mycobacterium tuberculosis (Mtb) infection.

Results: CD4CD8 T cells increase in frequency during early Mtb infection in PBMC and BAL from pre-infection. Peripheral, airway, and lung granuloma CD4CD8 T cells have distinct patterns and greater cytokine production than CD4 or CD8 T cells.

Conclusion: Our data suggest that CD4CD8 T cells transient the blood and airways early during infection to reach the granulomas where they are involved directly in the host response to Mtb.

Keywords: CD4CD8; T cells; mycobacteria; tuberculosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Disease Models, Animal
  • Granuloma / immunology
  • Leukocytes / immunology
  • Macaca fascicularis*
  • Mycobacterium tuberculosis / physiology
  • Tuberculosis / immunology*
  • Tuberculosis / microbiology