Development of Modular, Dual-Perfused Osteochondral Constructs for Cartilage Repair

Tissue Eng Part C Methods. 2019 Mar;25(3):127-136. doi: 10.1089/ten.TEC.2018.0356.

Abstract

This study describes methods for fabricating, culturing, and characterizing modular microbeads containing progenitor cells that can be used to create osteochondral tissue constructs. Such biphasic engineered tissues were cultured in a low flow rate perfusion bioreactor chamber to maintain tissue-specific differentiation while allowing development of the osteochondral interface.

Keywords: 3D cell culture; bioreactor; cartilage; hydrogels; mesenchymal stem cell; modular tissue engineering; osteochondral.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Newborn
  • Cartilage, Articular / cytology*
  • Cartilage, Articular / physiology
  • Cell Differentiation
  • Cells, Cultured
  • Chondrocytes / cytology*
  • Chondrocytes / physiology
  • Chondrogenesis*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / physiology
  • Osteogenesis*
  • Rats, Inbred F344
  • Tissue Engineering / methods*