Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide-Alkyne Click Chemistry in Physiological Conditions

Bioconjug Chem. 2019 Mar 20;30(3):531-535. doi: 10.1021/acs.bioconjchem.8b00868. Epub 2019 Feb 11.

Abstract

The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemical synthesis
  • Alkynes / chemistry
  • Azides / chemical synthesis
  • Azides / chemistry*
  • Click Chemistry / methods*
  • Cyclooctanes / chemical synthesis
  • Cyclooctanes / chemistry
  • Emulsions / chemical synthesis
  • Emulsions / chemistry
  • Immobilized Proteins / chemical synthesis
  • Immobilized Proteins / chemistry*
  • Polyesters / chemical synthesis
  • Polyesters / chemistry*
  • Serum Albumin, Human / chemical synthesis
  • Serum Albumin, Human / chemistry*

Substances

  • Alkynes
  • Azides
  • Cyclooctanes
  • Emulsions
  • Immobilized Proteins
  • Polyesters
  • polycaprolactone
  • Serum Albumin, Human