Crystal structure of AlpK: An essential monooxygenase involved in the biosynthesis of kinamycin

Wenpeng Wang; Jun Li; HuanHuan Li; Keqing Fan; Yingfang Liu

doi:10.1016/j.bbrc.2019.01.077

Crystal structure of AlpK: An essential monooxygenase involved in the biosynthesis of kinamycin

Biochem Biophys Res Commun. 2019 Mar 19;510(4):601-605. doi: 10.1016/j.bbrc.2019.01.077. Epub 2019 Feb 7.

Authors

Wenpeng Wang¹, Jun Li², HuanHuan Li¹, Keqing Fan³, Yingfang Liu⁴

Affiliations

¹ National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
² School of Medicine, Sun Yat-Sen University, Shenzhen, 510080, China.
³ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
⁴ National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, the Sixth Affiliated Hospital, School of Medicine, Sun Yat-sen University, Guangzhou, China. Electronic address: [email protected].

PMID: 30739782
DOI: 10.1016/j.bbrc.2019.01.077

Abstract

AlpK is an essential monooxygenase involved in the biosynthesis of kinamycin. It catalyzes the C5-hyfroxylattion of the crucial benzo[b]-fluorence intermediate in kinamycin synthesis. However, the structure and mechanism of AlpK is unclear. Here, we report the first structure of AlpK in complex with FAD. Our structure sheds light on the catalytic mechanism of AlpK.

Keywords: AlpK; Crystal structure; Kinamycin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism
Crystallography, X-Ray
Mixed Function Oxygenases / chemistry*
Mixed Function Oxygenases / metabolism
Models, Molecular
Protein Conformation
Protein Multimerization
Quinones / metabolism
Sequence Alignment
Streptomyces / chemistry
Streptomyces / enzymology*
Streptomyces / metabolism

Substances

Bacterial Proteins
Quinones
kinamycin A
Mixed Function Oxygenases